Optimization of Crosslinked Peptide Analysis on an Orbitrap Fusion Lumos Mass Spectrometer
نویسندگان
چکیده
Figure 3. Comparison of BSA crosslinking efficiency by SDS-PAGE. Different crosslinkers were incubated with BSA at molar excess of crosslinker to protein (e.g. 20, 100 or 500-fold). Crosslinking efficiency is shown by decreased mobility by SDS-PAGE and varied by crosslinker type, solubility and concentration. Figure 1. Structures of non-cleavable and MS-cleavable crosslinkers used for protein – protein interaction analysis.
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